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pcat-basic plasmid carrying the chloramphenicol acetyltransferase (cat) gene as a reporter  (Promega)

 
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    Structured Review

    Promega pcat-basic plasmid carrying the chloramphenicol acetyltransferase (cat) gene as a reporter
    Pcat Basic Plasmid Carrying The Chloramphenicol Acetyltransferase (Cat) Gene As A Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chloramphenicol+acetyltransferase+(cat)+gene+reporter+plasmid+(pcat-basic)/chloramphenicol+acetyltransferase/pm12163028-58-24-26
    Average 90 stars, based on 1 article reviews
    pcat-basic plasmid carrying the chloramphenicol acetyltransferase (cat) gene as a reporter - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Sequencing:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Polymerase Chain Reaction:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Clone Assay:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Construct:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Generated:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Subcloning:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).



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